#Chicken goat antibody cross reactivity license
Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license under any patent of ImmunoReagents, Inc._x000D_ _x000D_ Product may not be resold or modified for resale without prior written approval of ImmunoReagents, Inc._x000D_ĭyLightî is a trademark of Thermo Fisher Scientific, Inc. To minimize cross-reactivity, these chicken anti-goat IgG (H+L) whole secondary antibodies have been affinity purified and cross-adsorbed against human, mouse, and rabbit IgG prior to conjugation. non-immunoglobulin goat serum immunoglobulinsĬhicken Ig fraction was prepared using eggs from healthy hens of US origin.įor in vitro Laboratory Use Only.light chains on all goat immunoglobulinsīased on IEP, no reactivity is observed to: ÃÂ.For extended storage after rehydration, add an equal volume of glycerol and store at -20ÃÂ☌.īased on IEP, this antibody reacts with: àenables the detection of multiple antigens in the same sample, as well as the characterization of the primary antibody specificity and cross-reactivity. Product is stable for up to 4 weeks at 2-8ÃÂ☌ after rehydration. Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve.
#Chicken goat antibody cross reactivity free
Anti-Domestic ferret secondary antibodiesĬhicken anti-Goat IgG (H&L), Affinity PureĬhicken anti-Goat IgG (H&L) - Affinity Pure, DyLight®350 ConjugateĪffinity purified using solid phase Goat IgGĪffinity purified antibody is > 95% based on SDS-PAGEġ0 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free Immunological cross-reactions and species-specificities among bovine (BSA), goat (GSA), sheep (SSA), pig, equine, dog and human serum albumines were investigated with pooled rabbit antibodies to BSA, GSA and SSA by different immunochemical techniques. This immunoconJugate is not species-specific since Inter-species cross-reactivity is a normal feature of antisera to immunoglobulins.Anti-Rabbit & anti-Mouse secondary antibodies No cross-reaction between human -La and antibodies against bovine -La was detected.Here we focused on an overlooked cross immunoreactivity issue where a secondary antibody (secondary) cross-reacts with a primary antibody (primary) from a different species. Minimum cross-reactivity is observed with human. Immunostaining is a powerful technique and widely used to identify molecules in tissues and cells, although critical steps are necessary to block cross-reaction. A monoclonal antibody-based ELISA assay permitted the detection of chicken and. Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins based on immunoelectrophoresis. However, a better choice for multiple labeling is Rhodamine Red-X because its fluorescence is midway between a green fluorescing dye (like Alexa Fluor® 488) and a far-red-fluorescing dye like Alexa Fluor® 647. DNA analysis relying on polymerase chain reaction (PCR) and amplification. C圓 can also be paired with Alexa Fluor® 647 for multiple labeling when using a confocal microscope. C圓 has been used with fluorescein for double labeling however, the use of a narrow band-pass emission filter for fluorescein is recommended to minimize C圓 fluorescence in the FITC filter set.
C圓 can be excited to about 50% of maximum with an argon laser (514 nm or 528 nm lines), or to about 75% of maximum with a helium/neon laser (543 nm line) or mercury lamp (546 nm line). To minimize cross-reactivity, the antibody has been adsorbed against bovine, goat, guinea. We recommend C圓 as a brighter alternative to TRITC. Far-red fluorescent CF 647 dye has excitation/emission at 650/665 nm. For fluorescence microscopy, C圓 can be visualized with traditional tetramethyl rhodamine (TRITC) filter sets, since the excitation and emission spectra are nearly identical to those of TRITC. C圓 conjugates can be excited maximally at 550 nm, with peak emission at 570 nm. These goat anti-chicken IgY (H+L) whole secondary antibodies have been affinity-purified and show minimum cross-reactivity. C圓 is brighter, more photostable, and gives less background than other orange-red fluorescing dye conjugates.
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